New Study: Topical Cetirizine For Androgenetic Alopecia Shows Promise

[jamesbooker1975]

@jamesbooker1975 I created a 0.6% solution by crushing 30 10mg pills adding it to 30ml of distilled water and mixing it with 30ml of the childrens .1% solution. I think it’s getting systemically absorbed though because i put it on at night before i go to sleep. When I wake up I’m tired, drowsy and ywaning for the entire day and I get the dry nose. Do you get drowsiness sides at all? I want to keep on using it but not with these sides i’m getting

No,none side effects. An I use 2ml of the 1 % oral drops.

 

[Dimmer97]

Has any company pick this up and start trialing it?

 

[Ollie]

Has any company pick this up and start trialing it?

Why would they ? It’s a drug that’s already available .

 

[hope to success]

Is it effective to use cetirizine locally?

 

[jamesbooker1975]

Is it effective to use cetirizine locally?

Dude, did you read the study ? Yes, it is.

 

[Arden1]

Dude, did you read the study ? Yes, it is.

I've been using topical cetirizine for about a year. I dissolve it in water, apply to scalp, and then apply alcohol. It seems to only be effective for a week or so after I dissolve it. Then, it seems to degrade, but not sure. Do you have any experience dissolving it in water?

 

[jamesbooker1975]

I've been using topical cetirizine for about a year. I dissolve it in water, apply to scalp, and then apply alcohol. It seems to only be effective for a week or so after I dissolve it. Then, it seems to degrade, but not sure. Do you have any experience dissolving it in water?

but from where do you get that it is degrade it after a week ? IN the study they use ethanol.

 

[badhabiz]

I've been using topical cetirizine for about a year. I dissolve it in water, apply to scalp, and then apply alcohol. It seems to only be effective for a week or so after I dissolve it. Then, it seems to degrade, but not sure. Do you have any experience dissolving it in water?

experience after a year?

 

[HairOnFire]

Effect of Levocetirizine Hydrochloride on the Growth of Human Dermal Papilla Cells: A Preliminary Study

Ann Palliat Med 2020 Feb 28[Online ahead of print]

Abstract
Background: To conduct an in vitro investigation into the effect of different concentrations of levocetirizine hydrochloride on the growth of human dermal papilla cells (hDPCs) the underlying mechanisms involved.

Methods: hDPCs were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing different concentrations of levocetirizine hydrochloride for 48 h. The growth of hDPCs was observed by immunofluorescence staining, and the cell proliferation was detected by MTT assay. After the hDPCs were cultured in DMEM containing 1, 10, 100, 1,000, and 10,000 ng/mL levocetirizine hydrochloride for 48 h, the mRNA expressions of cyclooxygenase 2 (COX-2), prostaglandin D2 synthase (PTGDS), prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α), G protein-coupled receptor 44 (GPR44), protein kinase B (AKT), and glycogen synthase kinase 3β (GSK3β) were determined by real-time fluorescence-based quantitative polymerase chain reaction (PCR), and the protein expressions of PTGDS, phosphorylated protein kinase B (pAKT), and phosphorylated glycogen synthase kinase 3β (pGSK3β) were detected by Western blotting. After the hDPCs were cultured in DMEM containing 1, 10, 100, 1,000, 10,000 ng/mL levocetirizine hydrochloride for 24 h, the secretion levels of prostaglandin D2 (PGD2) and PGD2 receptor (PGD2R) in the culture supernatant were determined by enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance (ANOVA) was performed using SPSS 17.0 software, and the LSD-t test was used for pairwise comparisons.

Results: Immunofluorescence staining showed that hDPCs in the 100 ng/mL group grew well, with over 90% confluency. Methyl thiazolyl tetrazolium (MTT) method showed that the proliferation rate of hDPCs significantly differed between different levocetirizine hydrochloride groups and the blank control group (F=42.22, P<0.05), while the proliferation rate was significantly higher in the 100 ng/mL group (115.80%±5.10%) than in the blank control group (100%) (t=28.26, P<0.05). The relative mRNA expressions of COX-2, PGF2a, PTGDS, GPR44, and AKT showed significant differences in different levocetirizine hydrochloride groups (the F values were 1.97, 3.66, 2.17, 2.66, and 7.32, respectively; all P<0.05), whereas the mRNA expressions of PGE2 and GSK3β showed no significant difference (F=0.87, F=1.19, respectively; both P>0.05). The mRNA expressions of COX-2, PTGDS, and GPR44 in the 100 ng/mL group (0.840.08, 0.810.10, and 0.85±0.09, respectively) were significantly lower than those in the blank control group (t=1.97, t=2.17, and t=2.65, respectively; all P<0.05), whereas the mRNA expressions of PGF2α and AKT in the 100 ng/mL group (1.96±0.25 and 1.74±0.32, respectively) were significantly higher than those in the blank control group (t=3.662 and t=7.325, respectively; both P<0.05). There were significant differences in the levels of PTGDS, pAKT, pGSK3β, PGD2, and PGD2R proteins between the different levocetirizine hydrochloride groups (the F values were 11.84, 3.89, 4.07, 66.15, and 44.33, respectively). The protein expressions of PTGDS, PGD2, and PGD2R in the 100 ng/mL group (0.32±0.05, 141.62±5.44, and 215.08±9.55, respectively) were significantly lower than those in the blank control group (0.73±0.06, 180.08±6.15, and 273.24±3.18, respectively) (the t values were 5.66, 45.07, and 92.05, respectively; all P<0.05), whereas the protein expressions of pAKT and pGSK3β in the 100 ng/mL group (0.59±0.05 and 0.46±0.03, respectively) were significantly higher than those in the blank control group (0.46±0.02 and 0.35±0.042, respectively) (t=16.59, t=7.73, respectively; both P<0.05).

Conclusions: Levocetirizine hydrochloride may promote the growth and proliferation of hDPC in vitro by inhibiting the PGD2-GPR44 pathway and activating the AKT signaling pathway.

Keywords: Cetirizine; G protein-coupled receptor; alopecia; dermal papilla cells (DPCs); prostaglandin.

 

[HairCook]

Effect of Levocetirizine Hydrochloride on the Growth of Human Dermal Papilla Cells: A Preliminary Study

Ann Palliat Med 2020 Feb 28[Online ahead of print]

Abstract
Background: To conduct an in vitro investigation into the effect of different concentrations of levocetirizine hydrochloride on the growth of human dermal papilla cells (hDPCs) the underlying mechanisms involved.

Methods: hDPCs were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing different concentrations of levocetirizine hydrochloride for 48 h. The growth of hDPCs was observed by immunofluorescence staining, and the cell proliferation was detected by MTT assay. After the hDPCs were cultured in DMEM containing 1, 10, 100, 1,000, and 10,000 ng/mL levocetirizine hydrochloride for 48 h, the mRNA expressions of cyclooxygenase 2 (COX-2), prostaglandin D2 synthase (PTGDS), prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α), G protein-coupled receptor 44 (GPR44), protein kinase B (AKT), and glycogen synthase kinase 3β (GSK3β) were determined by real-time fluorescence-based quantitative polymerase chain reaction (PCR), and the protein expressions of PTGDS, phosphorylated protein kinase B (pAKT), and phosphorylated glycogen synthase kinase 3β (pGSK3β) were detected by Western blotting. After the hDPCs were cultured in DMEM containing 1, 10, 100, 1,000, 10,000 ng/mL levocetirizine hydrochloride for 24 h, the secretion levels of prostaglandin D2 (PGD2) and PGD2 receptor (PGD2R) in the culture supernatant were determined by enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance (ANOVA) was performed using SPSS 17.0 software, and the LSD-t test was used for pairwise comparisons.

Results: Immunofluorescence staining showed that hDPCs in the 100 ng/mL group grew well, with over 90% confluency. Methyl thiazolyl tetrazolium (MTT) method showed that the proliferation rate of hDPCs significantly differed between different levocetirizine hydrochloride groups and the blank control group (F=42.22, P<0.05), while the proliferation rate was significantly higher in the 100 ng/mL group (115.80%±5.10%) than in the blank control group (100%) (t=28.26, P<0.05). The relative mRNA expressions of COX-2, PGF2a, PTGDS, GPR44, and AKT showed significant differences in different levocetirizine hydrochloride groups (the F values were 1.97, 3.66, 2.17, 2.66, and 7.32, respectively; all P<0.05), whereas the mRNA expressions of PGE2 and GSK3β showed no significant difference (F=0.87, F=1.19, respectively; both P>0.05). The mRNA expressions of COX-2, PTGDS, and GPR44 in the 100 ng/mL group (0.840.08, 0.810.10, and 0.85±0.09, respectively) were significantly lower than those in the blank control group (t=1.97, t=2.17, and t=2.65, respectively; all P<0.05), whereas the mRNA expressions of PGF2α and AKT in the 100 ng/mL group (1.96±0.25 and 1.74±0.32, respectively) were significantly higher than those in the blank control group (t=3.662 and t=7.325, respectively; both P<0.05). There were significant differences in the levels of PTGDS, pAKT, pGSK3β, PGD2, and PGD2R proteins between the different levocetirizine hydrochloride groups (the F values were 11.84, 3.89, 4.07, 66.15, and 44.33, respectively). The protein expressions of PTGDS, PGD2, and PGD2R in the 100 ng/mL group (0.32±0.05, 141.62±5.44, and 215.08±9.55, respectively) were significantly lower than those in the blank control group (0.73±0.06, 180.08±6.15, and 273.24±3.18, respectively) (the t values were 5.66, 45.07, and 92.05, respectively; all P<0.05), whereas the protein expressions of pAKT and pGSK3β in the 100 ng/mL group (0.59±0.05 and 0.46±0.03, respectively) were significantly higher than those in the blank control group (0.46±0.02 and 0.35±0.042, respectively) (t=16.59, t=7.73, respectively; both P<0.05).

Conclusions: Levocetirizine hydrochloride may promote the growth and proliferation of hDPC in vitro by inhibiting the PGD2-GPR44 pathway and activating the AKT signaling pathway.

Keywords: Cetirizine; G protein-coupled receptor; alopecia; dermal papilla cells (DPCs); prostaglandin.

important to notice that too much in this study was detrimental and we talk about ng/ml:

 

[HairOnFire]

important to notice that too much in this study was detrimental and we talk about ng/ml: View attachment 139670

Good to know, thanks. When taken orally at the 5 mg dose, levocetirizine plasma concentrations are about 300 ng/mL.

 

[Pelopeleon]

Good to know, thanks. When taken orally at the 5 mg dose, levocetirizine plasma concentrations are about 300 ng/mL.

So, half a pill would be close to 150ng/l, not that far from that supposed good 100 ng/l

 

[killDHT]

nobody come back to report something? I think to use ceti in the coming time .Is here some people to tell me some advices?

 

[badhabiz]

nobody come back to report something? I think to use ceti in the coming time .Is here some people to tell me some advices?

Dont give up too easily. In my case shedding dropped after 3 weeks/a month

 

[killDHT]

Dont give up too easily. In my case shedding dropped after 3 weeks/a month

Thank you.do you use it nowaday?

 

[badhabiz]

Thank you.do you use it nowaday?

Yes. Same study carrier
2 ml daily

 

[ZenHead]

Dont give up too easily. In my case shedding dropped after 3 weeks/a month

What vehicle do you use? Water? Ethanol?

 

[badhabiz]

What vehicle do you use? Water? Ethanol?

cetirizine 1% , 16% cyclo-silicone-pentamer, ethyl alcohol

 

[killDHT]

cetirizine 1% , 16% cyclo-silicone-pentamer, ethyl alcohol

is so good .how long do you use it .and do you kept hair? thank you very much?

 

[devz]

Ok, I got diffuse thinning and inflammation on scalp, I stopped finasteride 10 months ago and now I'm losing my hair... Should I try it? I'm nw2/3 diffuse.