Does this study mean Dr Proctor is wrong about Nitric Oxide?

[donkdidonk2]

The Doctor says that NO stimulates regrowth and and I believe that NO is part of his NANO formulas. However, I have found this study:

Nitric oxide in the human hair follicle: constitutive and dihydrotestosterone-induced nitric oxide synthase expression and NO production in dermal papilla cells.

Wolf R, Schonfelder G, Paul M, Blume-Peytavi U.

Department of Dermatology, University Medical Center Benjamin Franklin, Free University of Berlin, Berlin, Germany.

The free radical nitric oxide, generated by different types of epidermal and dermal cells, has been identified as an important mediator in various physiological and pathophysiological processes of the skin, such as regulation of blood flow, melanogenesis, wound healing, and hyperproliferative skin diseases. However, little is known about the role of NO in the human hair follicle and in hair cycling processes. Here we demonstrate for the first time that dermal papilla cells derived from human hair follicles spontaneously produce NO by measuring nitrate and nitrite levels in culture supernatants. This biomolecule is apparently formed by the endothelial isoform of nitric oxide synthase, which was detected at the mRNA and protein levels. Remarkably, basal NO level was enhanced threefold by stimulating dermal papilla cells with 5alpha-dihydrotestosterone (DHT) but not with testosterone. Addition of N-[3-(aminomethyl)benzyl]acetamidine (1400W), a highly selective inhibitor of inducible nitric oxide synthase, restrained the elevation in NO level induced by DHT. Analyses of DHT-stimulated cells at the mRNA and protein levels confirmed the expression of inducible nitric oxide synthase. These findings suggest NO as a signaling molecule in human dermal papilla cells and implicate basal and androgen-mediated NO production to be involved in the regulation of hair follicle activity.

PMID: 12601527 [PubMed - indexed for MEDLINE]

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[Bryan]

What they didn't bother to tell you in that abstract (I've read the full study) is that they used OCCIPITAL human scalp hair follicles. It would have been fascinating to see the results for balding frontal or vertex follicles, but unfortunately they didn't choose do that testing. However, at least one of the authors' email addresses is included in the text, and I'm strongly tempted to write them and chastise them for what I consider to be an oversight, and ask them if they possibly have any unpublished information on that, or any speculation on the general role of nitric oxide in balding! :wink:

Bryan

 

[Petchsky]

I think you ought to...f*** it, why not.

 

[Armando Jose]

Hair loss affecting researchers?

Bryan wrote:

"What they didn't bother to tell you in that abstract (I've read the full study) is that they used OCCIPITAL human scalp hair follicles. It would have been fascinating to see the results for balding frontal or vertex follicles, but unfortunately they didn't choose do that testing. However, at least one of the authors' email addresses is included in the text, and I'm strongly tempted to write them and chastise (reprender) them for what I consider to be an oversight (descuido), and ask them if they possibly have any unpublished information on that, or any speculation on the general role of nitric oxide in balding!"

I am with you Bryan. The reason could be in the fact that common baldness don't affect to Mrs. Ulrike Blume-Peytavi. The same is the case of Mr. Tobin, her friend and another research in hair biology. In this study (*) he only used occipital hairs, and in the abstract appears:

It is shown here that minoxidil does not significantly increase hair shaft elongation or the duration of anagen VI in ex vivo culture despite several enhancements on the conventional methodology
Unexpectedly, minoxidil even inhibited hair shaft elongation in the absence of insulin.

I would like know if the results could be the same using vertex hairs. By the way, in my opinion there is not differences in healthy scalp hair.

OTOH, Bryan, I have not yet a response to your question regarding "luxuriant" hairs in CAIS. I am sorry.

Armando

(*) http://www.ncbi.nlm.nih.gov/entrez/quer ... s=15447724

Limitations of human occipital scalp hair follicle organ culture for studying the effects of minoxidil as a hair growth enhancer.

Magerl M, Paus R, Farjo N, Muller-Rover S, Peters EM, Foitzik K, Tobin DJ.

Department of Biomedical Sciences, University of Bradford, Bradford, UK.

 

[Armando Jose]

What is the real role of DHT in hair cycle?
http://www.ncbi.nlm.nih.gov/entrez/quer ... med_docsum

Important remark:
Remarkably, basal NO level was enhanced threefold by stimulating dermal papilla cells with 5alpha-dihydrotestosterone (DHT) but not with testosterone


NO is important:
The free radical nitric oxide, generated by different types of epidermal and dermal cells, has been identified as an important mediator in various physiological and pathophysiological processes of the skin, such as regulation of blood flow, melanogenesis, wound healing, and hyperproliferative skin diseases

Any input?

Armando